Publications
Books and Monographs
  1. Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy. Edited by Dan Simionescu and Agneta Simionescu, InTech (2017). Print ISBN 978-953-51-3023-9. Open Access.

  2. L Sierad, A Simionescu, D Simionescu. "Automated Heart Valve Bioreactors for Translational Research". Book chapter in Advances in Heart Valve Biomechanics: The Role of Mechanics in Valvular Physiology, Mechanobiology, and the Bioengineering of Repair and Replacement. Edited by M Sacks and J Liao, Springer, USA, (2016).

  3. Jeremy J. Mercuri, D. Simionescu, “Advances in Tissue Engineering Approaches to Treatment of Intervertebral Disc Degeneration: Cells and Polymeric Scaffolds for Nucleus Pulposus Regeneration”. By invitation. Review in Advances in Polymer Sciences, (Springer Berlin/Heidelberg), special volume on Polymers in Nanomedicine Volume 247, Pages: 201-231. Editors: Kunugi S, Yamaoka T. Kyoto, Japan, (2012). DOI: 10.1007/12_2011_149

  4. G Fercana, D Simionescu. “Polymeric Biomaterials for Vascular Tissue Engineering”. By invitation. In “Polymers for Vascular and Urogenital Applications”. Edited by Shalaby W. Shalaby, K. Burg, and W. Shalaby. Taylor & Francis USA, (2012). ISBN 978-1-4200-7694-3. DOI: 10.1201/b11853-4

  5. Dan Simionescu, Agneta Simionescu, book editors. InTech - Open Access Publisher, Rijeka, Croatia. Title, “Vasculogenesis and Angiogenesis – from Embryonic Development to regenerative Medicine”. ISBN 978-953-307-882-3. (November 2011). Open Access.

  6. Simionescu D. "Artificial heart valves ". By invitation; Encyclopedia of Biomedical Engineering, Metin Akay, editor. John Wiley and Sons, Inc., Hoboken, NJ. 2006. 

  7. Simionescu D, Simionescu A, Deac R, "Molecular pathology of biomaterials, in "Cell adhesion and the extracellular matrix". C. Cruce, A. Simionescu, D. Simionescu et.al., (eds), AIUS Publishers, Craiova, Romania, 1997. (in Romanian).

  8. R. Deac, D. Simionescu, "Heart biomechanics", In BIOMECANICS, 1989, Romanian Academy Press, Bucharest, pp.127-140. (in Romanian).

Refereed Journal Publications

     Article abstracts may be found online at Pubmed or located using the links below.
     Full text articles can be accessed using the PMCID links.

  1. F Alexis, M Casco, T Olsen, A Herbst, G Evans, T Rothermel, L Pruett, D Simionescu,  R Visconti, "Iron Oxide Nanoparticles Stimulates Extra-Cellular Matrix Production into Cellular Spheroids." (2017) Bioengineering, 4(1). Direct Link

  2. M Harpa, I Movileanu, L Sierad, O Cotoi, H Suciu, T Preda, D Nistor, C Sircuta, K Branzaniuc, R Deac, S Gurzu, L Harceaga, P Olah, D Simionescu, M Dandel, A Simionescu. “In vivo testing of xenogeneic acellular aortic valves seeded with stem cells”. (2016) Romanian Review of Laboratory Medicine, 24(3), 343-346. Direct Link

  3. C Deborde, D Simionescu, J Liao, L Sierad, C Wright, A Simionescu. “Stabilized collagen and elastin-based scaffolds for mitral valve tissue engineering”. (2016) Tissue Engineering, Part A. 22(21-22), 1241-1251. 27608885, PMC5107718

  4. A Kennamer, L Sierad, R Pascal, N Rierson, C Albers, M Harpa, O Cotoi, L Harceaga, P Olah, P Terezia, A Simionescu, D Simionescu. “Bioreactor conditioning of valve scaffolds seeded internally with adult stem cells”. (2016) Tissue Engineering and Regenerative Medicine, 13(5), 507-515. Abstract

  5. M Harpa, I Movileanu, L Sierad, O Cotoi, H Suciu, C Sircuta, T Preda, D Nistor, K Branzaniuc, R Deac, M Dandel, S Gurzu, L Harceaga, P Olah, A Simionescu, D Simionescu. “Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells”. (Jan. 2016) In press, Romanian Review of Laboratory Medicine. Abstract

  6. M Harpa, I Movileanu, H Suciu, C Sircuta, K Branzaniuc, S Gurzu, L Harceaga, P Olah, A Simionescu, D Simionescu. “Mechanisms of failure of stem cell-seeded acellular valved conduits in a sheep right ventricular outflow tract model”. (Dec. 2015) Submitted to Acta Cardiologica Sinica.

  7. I Koleilat, M Jaeggli, J Ewing, M Androes, D Simionescu, J Eidt. “Interobserver variability in physician-modified endograft planning by comparison with a three-dimensional printed aortic model”. (2016) J Vasc Surg, 64(6), 1789-1796. 26607872

  8. L Sierad, EL Shaw, A Bina, B Brazile, N Rierson, S Patnaik, A Kennamer, R Odum, O Cotoi, T Preda, K Branzaniuc, H Smallwood, R Deac, I Egyed, Z Pavai, A Szanto, L Harceaga, H Suciu, V Raicea, P Olah, A Simionescu, J Liao, I Movileanu, M Harpa, D. Simionescu. “Functional heart valve scaffolds obtained by complete decellularization of porcine aortic roots in a novel differential pressure gradient perfusion system”. (2015) Tissue Engineering, Part C,21(12), 1284-1296. 26467108, PMC4663650

  9. M Harpa, K Branzaniuc, I Movileanu, O Cotoi, H Suciu, R Deac, L Harceaga, A Simionescu, D Simionescu. “Implantation of valvular collagen biomaterials seeded with autologous stem cells – intraoperative hemodynamic measurements in an animal model”. (2015). Acta Medica Transylvanica, 20(2), 38-39. Abstract

  10. L. Roudsari, C. Albers, T. Doucet, S. Floyd, C. Hoffman, K. Leaphart, E. Patrick, B. Roach, Ml Mahoney, L Sierad, J Nagatomi, D Simionescu. “Gelatin-Coated Resorbable Polymer Mesh as a Novel Scaffold for Aortic Heart Valve Tissue Engineering” (2014). Challenges in Regenerative Medicine, 1(1), 39-45. Direct Link

  11. D Simionescu. “Educational Ethics in Regenerative Medicine; a Hippocratic Oath for Biomedical Engineers”. (2014). Challenges in Regenerative Medicine, 1(1), 37-38. Direct Link

  12. LN Sierad, EL Shaw, R Launius, S McBride, C Storholt, R Poole, D Spence, K Miller, L Sosdian, K Allen, L Burton, A Iari, J Nagatomi, D Simionescu. “Toward an Endothelial-Cell Covered Mechanical Valve; Surface Re-Engineering and Bioreactor Testing of Mechanical Heart Valves”. (2014). Challenges in Regenerative Medicine, 1(1), 22-34 Direct Link

  13. T Pennel, G Fercana, D Bezuidenhout, A Simionescu, T Chuang, P Zilla, D Simionescu. “The Performance of Cross-linked Acellular Arterial Scaffolds as Vascular Grafts; Pre-clinical Testing in Direct and Isolation Loop Circulatory Models”. (2014).Biomaterials.  24816365, PMC4048826

  14. G. Fercana, D. Bowser, M. Portilla , E.M. Langan, C.G. Carsten, D.L. Cull, L.N. Sierad , D.T. Simionescu. “Platform technologies for decellularization, tunic-specific cell seeding and in vitro conditioning of extended length, small diameter vascular grafts”. (2014). E-pub, in press, Tissue Engineering, Part C. 24749889, PMC4241864

  15. Thomas Larrew, James Chow , Jason Schulte , Dan Simionescu, Agneta Simionescu. “The roles of various metabolic enzymes in diabetic cardiomyopathy—in vivo and in vitro approach”. Cardiovascular Pathology, (2013); 22(3):e48. Abstract

  16. Mattix, B., Olsen, T., Casco, M., Herbst, A., Simionescu, D., Visconti, R., Alexis, F. “Biological magnetic cellular spheroids as building blocks for tissue engineering.” Acta Biomaterialia. (2014); 10(2): 623-629. 24176725, PMC4733563

  17. Mattix, B., Moore, T., Olsen, T., Simionescu, D., Visconti, R., Alexis, F. “Accelerated iron oxide nanoparticle degradation via polymer encapsulation for tissue engineering.” Advanced Functional Materials. (2014); 24(6): 800-807.  Abstract

  18. Mattix, B., Poole, J., Casco, M., Uvarov, O., Simionescu, D., Alexis, F. ”Small Diameter Blood Vessels: Replacement Strategies, Maturation Techniques, and Vascular Network Formation”. Journal of Tissue Science & Engineering, (2013), 4(2),1. Abstract

  19. George Fercana, Eugene Langan, Christopher Wright, Dan Simionescu. “Development of living vascular grafts for peripheral bypass applications using a novel trans-mural perfusion bioreactor”. Cardiovascular Pathology, (2013), 22(3):e35. Abstract

  20. JJ Mercuri, C Addington, R Pascal, S Gill, D Simionescu. "Development and initial characterization of a chemically stabilized elastin-glycosaminoglycan-collagen composite shape-memory hydrogel for nucleus pulposus regeneration." Journal of Biomedical Materials Research, Part A. Epub ahead of print. (2014). 24497431

  21. JP Chow, DT Simionescu, H Warner , B Wang, SS Patnaik, J Liao, A Simionescu. "Mitigation of Diabetes-Related Complications in Implanted Collagen and Elastin Scaffolds Using Matrix-Binding Polyphenol". Biomaterials, 34(3): 685-695 (2013) 23103157, PMC3496025

  22. JJ Mercuri, S Patnaik, SS Gill, J Liao, DT Simionescu. "Regenerative Potential of Decellularized Porcine Nucleus Pulposus Hydrogel Scaffolds; Stem cell Differentiation, Matrix Remodeling and Biocompatibility Studies". Tissue Engineering, Part A., 19(7-8): 952-66 (2013) 23140227

  23. JB Schulte, A Simionescu, DT Simionescu. "The Acellular Myocardial Flap; a Novel Extracellular Matrix Scaffold Enriched with Patent Microvascular Networks and Biocompatible Cell Niches". Tissue Engineering, Part C.,19(7): 518-30 (2013). 23151037, PMC3662386

  24. B Wang, ME Tedder, CE Perez, G Wang, AL de Jongh Curry, F To, SH Elder, LN Williams, DT Simionescu, J Liao. "Structural and Biomechanical Characterizations of Porcine Myocardial Extracellular Matrix". Journal of Materials Science: Materials in Medicine, 23(8):1835-1847 (2012). 22584822, PMC3523096

  25. Dan T. Simionescu, Joseph Chen, Michael Jaeggli, Jun Liao. “Form Follows Function: Advances in Trilayered Structure Replication for Aortic Heart Valve Tissue Engineering.” Journal of Healthcare Engineering, 3(2): 179-202 (2012) 23355946, PMC3552623

  26. Agneta Simionescu,  Jason Schulte, George Fercana and D. Simionescu. "Inflammation in Cardiovascular Tissue Engineering: the Challenge to a Promise". International Journal of Inflammation. (2011). 21755031, PMC3132660

  27. Agneta Simionescu, Mary E. Tedder, Ting-Hsien Chuang, D. Simionescu. “Lectin and Antibody-Based Histochemical Techniques For Cardiovascular Tissue Engineering”. Journal of Histotechnology, 34(1): 20-28 (2011). Abstract

  28. Tedder ME, Liao J, Simionescu A, Simionescu DT, “Assembly and testing of layered collagen constructs for heart valve tissue engineering”. Tissue Engineering, Part A, 17(1-2): 25-36. (2011). 20673028, PMC3011922

  29. Jeremy J. Mercuri,Sanjitpal S. Gill, Dan T. Simionescu, “Novel Tissue Derived Biomimetic Scaffold for Regenerating the Human Nucleus Pulposus”. Journal of Biomedical Materials Research, part A. Feb, 96A(2): 422–435. (2011). 21171162

  30. M.B. Ariganello, D. Simionescu, R.S. Labow, J. Michael Lee, “Macrophage Differentiation and Polarization on a Decellularized Pericardial Biomaterial”. Biomaterials, 32(2): 439-49 (2011). 20933269, PMC3568399

  31. Jeremy J. Mercuri , Sanjitpal S. Gill, D. Simionescu, “Novel Tissue-Derived Biomimetic Scaffolds for Regenerating the Human Nucleus Pulposus”, e-pub, Journal of Biomaterial Materials Research, part A., 96(2): 422-35 (2011). 21154457

  32. Leslie N. Sierad, Christopher Albers, Jordan Maivelett, Mary E. Tedder, Jun Liao, D. Simionescu, Agneta Simionescu, “Endothelialization of decellularized penta-galloyl-glucose treated xenograft scaffolds and dynamic conditioning in a heart valve bioreactor”, Cardiovascular Engineering and Technology, 1(2): 138-153 (2010).

  33. Bo Wang, Ali Borazjani, Mina Tahai, Amy L. de Jongh Curry, D. Simionescu, Jianjun Guan, Filip To, Steve H. Elder, Jun Liao, “Fabrication of Cardiac Patch with Decellularized Porcine Myocardial Scaffold and Bone Marrow Mononuclear Cells”, Journal of Biomaterial Materials Research, part A., 94(4): 1100-1110 (2010). 20694977, PMC2933781

  34. M. E. Plonska-Brzezinska, A. Palkar, K. Winkler, A. Simionescu, D. Simionescu, A. Dubis, A. J. Athans, L. Echegoyen, “Small non-cytotoxic carbon nano-onions: investigation of the electrochemical behavior and their potential application”, Chemistry, 16(16): 4870-4880. (2010).

  35. Jeremy J. Mercuri, Sanjitpal S. Gill, D. Simionescu, “Novel Tissue-Derived Biomimetic Scaffolds for Regenerating the Human Nucleus Pulposus”, Journal of Biomaterial Materials Research, part A., 96(2): 422-35 (2011). 21171162

  36. T. Chuang, C. Stabler, A. Simionescu, D. Simionescu, “Polyphenol-stabilized tubular elastin scaffolds for tissue engineered vascular grafts”, Tissue Engineering Part A, 15(10): 2837-2851 (2009). 19254115, PMC2792047

  37. M. E. Tedder, J. Liao, B. Weed, C. Stabler, H. Zhang, A. Simionescu, D. Simionescu, “Stabilized collagen scaffolds for heart valve tissue engineering”, Tissue Engineering Part A, 15(6): 1257-1268 (2009). 18928400, PMC2792094

  38. Y.Y. Yuan, P.J. Kostenuik, S. Morony, D. Simionescu, D.M. Basalyga, T.A. Bateman, “Skeletal deterioration induced by RANKL infusion; a model for high-turnover bone disease”, Osteoporosis International, 19(5): 625-35 (2008). 18038244

  39. Simionescu A, Simionescu D, Vyavahare N, “Osteogenic responses in fibroblasts activated by elastin degradation products and transforming growth factor-beta1: role of myofibroblasts in vascular calcification”, American Journal of Pathology, 171(1): 116-23 (2007). 17591959, PMC1941602

  40. Raghavan D, Simionescu D, Vyavahare N, “Neomycin prevents enzyme-mediated glycosamino-glycan degradation in bioprosthetic heart valves”, Biomaterials, 28(18): 2861-8 (2007). 17353047, PMC2262162

  41. Kurane A, Simionescu D, Vyavahare N, “In vivo cellular repopulation of tubular elastin scaffolds mediated by basic fibroblast growth factor”, Biomaterials, 28(18): 2830-2838 (2007). 17368531, PMC2262161

  42. Isenburg J, Simionescu D, Starcher B, Vyavahare N, “Elastin stabilization for treatment of abdominal aortic aneurysms”, Circulation, 115(13): 1729-37 (2007). 17372168. Full Text

  43. Mercuri J, Lovekamp J, Simionescu D, Vyavahare NR, “Glycosaminoglycan-targeted fixation for improved bioprosthetic heart valve stabilization”, Biomaterials, 28(3): 496-503 (2007). 17030363

  44. Simpson CL, Lindley S, Eisenberg C, Basalyga DM, Starcher BC, Simionescu D, Vyavahare NR, “Toward cell therapy for vascular calcification: osteoclast-mediated demineralization of calcified elastin”, Cardiovascular Pathology, 16(1): 29-37 (2007). 17218212

  45. Kasyanov V, Isenburg JC, Draughn AR, Hazard S, Hodde J, Iveta O, Halkes B, Pieters R, Simionescu D, Markwald RR, Mironov V, “Tannic acid mimicking dendrimers as a small intestine submucosa stabilizing nano-agent”, Biomaterials, 27(5): 745-751 (2006). 16102811

  46. Isenburg JC, Simionescu D, Ramachandani N, Vyavahare NR, “Structural requirements for stabilization of vascular elastin by polyphenolic tannins”, Biomaterials, 27(19): 3645-3651 (2006). 16527345

  47. Lee JS, Basalyga D, Simionescu D, Vyavahare NR, “Elastin calcification in the rat subdermal model is accompanied by up-regulation of degradative and osteogenic cellular responses”, American Journal of Pathology, 168(2): 490-498 (2006). 16436663, PMC1606489

  48. Isenburg J, Simionescu D, Vyavahare N, "Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall", Biomaterials, 26(11): 1237-1245 (2005). 15475053

  49. Lovekamp JJ, Simionescu D, Mercuri J, Zubiate B, Sacks M, Vyavahare NR, “Stability and function of glycosaminoglycans in porcine bioprosthetic heart valves”, Biomaterials, 27 (8): 1507-1518 (2005). 16144707, PMC2262164

  50. Simionescu D, Lu Q, Song Y, Lee JS, Rosenbalm TN, Kelley C, Vyavahare NR, "Biocompatibility and remodeling potential of pure arterial elastin and collagen scaffolds", Biomaterials, 27 (5), 702-713 (2005). 16048731

  51. Isenburg JC, Simionescu D, Vyavahare NR, "Elastin stabilization in cardiovascular implants: improved resistance to enzymatic degradation by treatment with tannic acid", Biomaterials, 25(16): 3293-3302 (2004). 14980424

  52. Bailey M, Pillarisetti S, Jones P, Xiao H, Simionescu D, Vyavahare N, "Involvement of matrix metalloproteinases and tenascin-C in elastin calcification", Cardiovascular Pathology, 13(3): 146-155 (2004). 15081471

  53. Lu Q, Ganesan K, Simionescu D, Vyavahare NR, "Novel porous aortic elastin and collagen scaffolds for tissue engineering", Biomaterials, 25(22): 5227-5237 (2004). 15110474

  54. Basalyga DM, Simionescu D, Xiong W, Baxter BT, Starcher BC, Vyavahare NR, "Elastin degradation and calcification in an abdominal aorta injury model: role of matrix metalloproteinases", Circulation, 110(22): 3480-3487 (2004). 15545515,
    PMC1262646


  55. Simionescu D, "Prevention of calcification in bioprosthetic heart valves: challenges and perspectives". By invitation. Expert Opinions in Biological Therapy, 4(12), 1971-1985 (2004). 15571459

  56. Simionescu D, Lovekamp JJ, Vyavahare NR, "Extracellular matrix degrading enzymes are active in porcine stentless aortic bioprosthetic heart valves". Journal of Biomedical Materials Research, 66(4): 755-763 (2003). 12926026

  57. D. Simionescu, J. Lovekamp, N. Vyavahare, “Glycosaminoglycan-degrading enzymes in porcine aortic heart valves; implications for bioprosthetic heart valve degeneration". Journal of Heart Valve Disease, 12(2): 217-225 (2003). 12701795

  58. D. Simionescu, J. Lovekamp, N. Vyavahare, “Degeneration of stentless bioprosthetic heart valves is initiated during tissue preparation; an ultrastructural study. Journal of Heart Valve Disease, 12(2): 226-234 (2003). 12701796

  59. M. Bracher, D. Simionescu, A. Simionescu, N. Davies, P. Human, P. Zilla, “The role of matrix metalloproteinases in tissue valve degeneration – a mini-review”. Journal of Long Term Effects of Medical Implants, 11(3-4): 221-230 (2001).

  60. A. Simionescu, D. Simionescu, R. Deac, "Biochemical pathways of tissue degeneration in bioprosthetic cardiac valves; the role of matrix metalloproteinases". ASAIO Journal, 42(5): M561-M567 (1996). 8944942

  61. A. Simionescu, D. Simionescu, R. Deac, "Matrix metalloproteinases in the pathology of natural and bioprosthetic cardiac valves". Cardiovascular Pathology, 5(6): 323-332 (1996). Abstract

  62. R. Deac, D. Simionescu, D. Deac, "New evolution in mitral physiology and surgery; mitral stentless pericardial valve", Annals of Thoracic Surgery, 60(2 Supp): S433-S438 (1995). 7646203

  63. D. Simionescu, R. Deac, A. Simionescu, "Inhibition of extracellular-matrix degrading proteases in pericardial derived cardiovascular bioprostheses", Journal of Material Science; Materials in Medicine, 5(9-10): 748-751 (1994). Abstract

  64. D. Simionescu, A. Simionescu, R. Deac, "Remnant proteolytic ac­tivities in glutaraldehyde treated bovine pericardium and in ex­planted cardiac bioprostheses", Journal of Biomedical Materials Research, 27(6): 821-829 (1993). 8408112

  65. D. Simionescu, A. Simionescu, R. Deac. "Mapping of glutaraldehyde-treated bovine pericardium for use as bioprosthetic cardiac valves", Journal of Biomedical Materials Research,27 (6): 697-704 (1993). 8408100

  66. Simionescu A, D. Simionescu, R. Deac. "Lysine-enhanced glutaral­dehyde crosslinking of collagenous biomaterials", Journal of Biomedical Materials Research, 25(12): 1495-1505 (1991). 1794997

  67. D. Simionescu, N.A.  Kefalides. "Biosynthesis of proteoglycans and interstitial collagens by bovine pericardial fibroblasts", Experimental Cell Research, 195(1), 171-176 (1991). 2055265

  68. D. Simionescu, R.V. Iozzo and N.A. Kefalides. "Bovine pericardial proteoglycan; biochemical, immunochemical and ultrastructural studies", Matrix, 9(4): 301-310 (1989). 2677626

  69. D. Simionescu, R. Alper and N.A. Kefalides. "Partial characterization of a low molecular weight proteogly­can isolated from bovine parietal pericardium". Biochemical and Biophysical Research Communications, 151(1): 480-486 (1988). 3348789

  70. D. Simionescu, M. Simionescu. "Differentiated distribution of the cell surface charge on the alveolar capillary unit".  Microvascular Research, 25(1): 85-100 (1983). 6835101